The possibilities of the use of N-of-1 and do-it-yourself trials in nutritional research

by Tanja Krone, Ruud Boessen, Sabina Bijlsma, Robin van Stokkum, Nard D. S. Clabbers, Wilrike J. Pasman

Background

N-of-1 designs gain popularity in nutritional research because of the improving technological possibilities, practical applicability and promise of increased accuracy and sensitivity, especially in the field of personalized nutrition. This move asks for a search of applicable statistical methods.

Objective

To demonstrate the differences of three popular statistical methods in analyzing treatment effects of data obtained in N-of-1 designs.

Method

We compare Individual-participant data meta-analysis, frequentist and Bayesian linear mixed effect models using a simulation experiment. Furthermore, we demonstrate the merits of the Bayesian model including prior information by analyzing data of an empirical study on weight loss.

Results

The linear mixed effect models are to be preferred over the meta-analysis method, since the individual effects are estimated more accurately as evidenced by the lower errors, especially with lower sample sizes. Differences between Bayesian and frequentist mixed models were found to be small, indicating that they will lead to the same results without including an informative prior.

Conclusion

For empirical data, the Bayesian mixed model allows the inclusion of prior knowledge and gives potential for population based and personalized inference.