SDS-PAGEStellen Sie ein SDS-PAGE-Gel entsprechend dem Molekulargewicht (MW) Ihrer Zielproteine her. (Rezepte finden Sie im Abschnitt "SDS-PAGE-Gelrezepte")Bereiten Sie Proben im Mikrozentrifugenröhrchen vor. Fügen Sie vier Mal Probenpuffer hinzu, so dass die Gesamtproteinmenge etwa 30 - 50 ug je Probe beträgt (entsprechend der Proteinmenge, die durch den Bradford- oder BCA-Test gemessen wird).Flicken Sie die Mikrozentrifugenröhrchen um die Proben zu mischen, drehen Sie sie kurz und erwärmen Sie sie... Читать дальше...
There was an understandable fear that 2024 would be a grim year for the movies, but it turned out that 2024 was really just a grim year for everything else. Phew!Of course, some concerns about the state of the film industry did come to fruition. The strikes threatened to significantly reduce the number of movies that Hollywood was able to release in theaters, and there were indeed several weeks in which consumers were left with little to choose from at their local multiplex. That crisis was inexplicably... Читать дальше...
We’ve finally arrived at the end of 2024, and what a year it's been. We’ve been treated to all kinds of cinematic goodness since January: there’s been space-bound sequels and dystopian prequels; wholly original, genre-blending flexes from some of the most exciting auteurs working today; emotional animated adventures; heartbreaking historical biopics; bloodcurdling body horrors; quite literally hundreds of beavers; and more.And so, as we reflect on the year that was, we here at Team Empire have corralled our editors... Читать дальше...
Photograph: Time OutFrom The Zone of Interest to Wicked, the very best reasons to head to the cinema this yearWednesday 4 December 2024Edited by Phil de SemlyenGlobal film editorContributors: Anna Smith, Olly Richards, Stephen A Russell, David Hughes, Hanna Flint & Matthew SingerAdvertisingIt started slowly but 2024 came through for us at the cinema – and, to a lesser extent, on streaming. Nothing quite matched the cultural behemoth that was Barbenheimer – much as noble marketing folk tried to... Читать дальше...
This has been a difficult year. You may have been buffeted by anger you didn’t want to feel, and didn’t know how to productively process. Breeding grounds for misinformation—and its even more treacherous sibling, disinformation—have proliferated. AI is coming, supposedly to make our lives easier, but in reality it may just be taking our jobs. Sometimes it has seemed that soullessness has become the order of the day—as if having a soul were simply too much bother.But as of right now, at least, most movies are made by human beings... Читать дальше...
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Samsung betting on Apple to make the Galaxy phones more popular PhoneArena
In a bizarre incident, a man chopped off four fingers of his left hand with a sharp knife to make himself unfit for his job as a computer operator at his relative's diamond firm, police in Gujarat's...
Ahead of his Test retirement, New Zealand pace talisman Tim Southee was given a memorable final entrance.
A member of the House of Representatives, representing Ogbomoso North, South and Oriire Federal Constituency in Oyo State, Olamijuwonlo Alao-Akala, has reiterated the commitment of the National Assembly to enacting laws that enhance youth involvement in policy-making and national development. He made this known during the inauguration of a state-of-the-art facility donated by Seyi Tinubu
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Former Union Minister Suresh Prabhu, speaking at IRMA's 45th Foundation Day, emphasized people-centric development over infrastructure-driven growth for India's transformation into the third-largest economy. He highlighted Dr. Verghese Kurien's cooperative model as an inspiration, advocating for aligning GDP growth with rising per capita income and climate-proofing agriculture for a developed India by 2047.
Women are often told to shrug things off but this type of behaviour rarely happens in isolation, writes Olivia Petter. If we don’t take it more seriously, who knows where it may lead?
BUFFALO, N.Y. (WIVB) -- One woman from Western New York was part of the largest single-day act of clemency in U.S. history on Thursday when President Joe Biden granted pardons and commutations to 1,500 people. “It’s really an acknowledgment that we, you know, behaved ourselves and clearly made some changes and acted right,” Wendy Lankton [...]
Some San Francisco residents were jolted awake by a tornado warning early Saturday.
So it seems that in addition to more money ever paid to any player in any sport ever, Juan Soto gets possession of No. 22 written into his contract. Rules
Pop sensation Taylor Swift celebrated her 35th birthday yesterday (13 December) and among her many fans, NFL wished her with a heartwarming video that left her fans ‘sobbing’.
He was found guilty on a charge of 'collaborating with the hostile U.S. government'
Putting tomato ketchup on a traditional roast turkey Christmas dinner is the biggest festive 'ick' in Britain.
Ryan Reynolds and Channing Tatum were joined by a third celebrity as they arrived at The Fat Boar in Wrexham for a fun-fuelled Friday night - with the Deadpool star buying drinks for all
