It was the only one of the three Group One prizes on offer to be won by a foreign raider -- Rosallion and Charyn winning the St James's Palace and Queen Anne Stakes respectively.
Asfoora's jockey Oisin Murphy's joy at winning knew no bounds.
The 28-year-old stood up in the saddle and waved his whip at the stands as top-hatted men and women, dressed in the latest fashions, cheered and applauded the popular Irishman.
Dwyer had studied accountancy at university and said he would do something else if he failed to make it as a trainer -- Tuesday's victory ensures he will not need to give up the day job.
"We've come a long way from Ballarat," said Dwyer.
"It was a bit of an audacious plan. We got a bit of stick at home for bringing her over because she wasn't seen as one of our better sprinters."
Dwyer said his other owners would be keen to have a go next year.
"There is another group of 40 or 50 of my owners from home that would saw their left leg off to have a runner at Royal Ascot," he said.
Asfoora's owner and breeder Akram El-Fahkri has had quite a journey to receiving the tophy from King Charles III -- the taxi firm owner traces his love of racing back to growing up on his parents farm in Lebanon.
The St James's Palace Stakes brought together the winners of the English, Irish and French 2000 Guineas.
It was to be Rosallion, the winner of the Irish 2000 Guineas who won in impressive fashion, beating Henry Longfellow with French Guineas victor Metropolitan third.
English 2000 Guineas champion Notable Speech -- who had Rosallion back in second at Newmarket -- never fired and finished well beaten.
"Probably the most satisfactory win of my career," said a tearful winning trainer Richard Hannon Junior, recording his ninth Royal Ascot winner and second in the race.
Winning jockey Sean Levey, triumphing only for the second time at the meeting, said Rosallion is the 'Declan Rice' (England midfielder) of racing.
"Boy was he good," said Levey.
"It means the world to me. I always wanted to ride a horse that was that good that people would associate my name with his."
'A great day'
Earlier, Silvestre de Sousa's excellent season on his return from Hong Kong continued as the Brazilian jockey guided Charryn home in the Group One Queen Anne Stakes.
The 43-year-old three-time British champion jockey -- who won his first classic earlier this season on Elmalka in the English 1000 Guineas -- remained patient as Audience set the pace.
De Sousa -- who served a 10-month ban for a betting offence committed when he was riding in Hong Kong last year -- then produced Charryn with a perfectly-timed challenge and he eased to victory.
It ended Charryn's Group One drought, finally winning one at the eighth attempt.
It was de Sousa' eighth winner at Royal Ascot but his first Group One.
"Absolutely, a great season," he said.
"Sometimes I have to pinch myself to believe what is going on."
Trainer Roger Varian said it would be hard to top the feeling.
"It does not get any better than this, winning a Group One and the first race at Royal Ascot," he said.
"I am delighted for the owner Nurlan Bizakov, who has invested so much in the game and supported me for so many years. It is a great day."
The bookmakers wore the brightest grins after the second race as last year's champion apprentice jockey Billy Loughnane guided home 80/1 outsider Rashabar to a shock win in the Group Two Coventry Stakes.
"You grow up watching races like this," said 18-year-old Loughnane, who only got the ride as Levey was claimed for another horse.
"It's always been a dream of mine to try and win one, it's my first Group race in the UK and it means a lot."
