Nucleic acids analysis is mainly used in pathogen detection, genetic disease identification and early cancer diagnosis. For example, quantitative analysis of circulating tumor DNA (ctDNA), a free DNA fragment derived from malignant cells which carries tumor specific sequence changes, can help obtain abundant information about tumors, including gene point mutation, genome integrity. Therefore, ctDNA is considered as a personalized tumor marker and plays a key role in cancer diagnosis and malignancy evaluation.
